NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 754.8
Mean read quality 10.8
Median read length 421.0
Median read quality 12.2
Number of reads 30,474.0
Read length N50 1,690.0
STDEV read length 882.3
Total bases 23,003,180.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 30178 (99.0%) 23.0Mb
>Q7 29428 (96.6%) 22.9Mb
>Q10 23731 (77.9%) 18.3Mb
>Q12 16020 (52.6%) 10.8Mb
>Q15 5392 (17.7%) 0.5Mb
Top 5 highest mean basecall quality scores and their read lengths
1 35.9 (17)
2 34.9 (16)
3 34.8 (16)
4 34.6 (16)
5 34.6 (18)
Top 5 longest reads and their mean basecall quality score
1 7728 (12.5)
2 7186 (11.3)
3 6587 (13.0)
4 6397 (10.4)
5 6225 (11.9)

Summary statistics after filtering

General summary
Mean read length 758.2
Mean read quality 10.8
Median read length 429.0
Median read quality 12.2
Number of reads 30,336.0
Read length N50 1,691.0
STDEV read length 882.9
Total bases 23,000,918.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 30040 (99.0%) 23.0Mb
>Q7 29290 (96.6%) 22.9Mb
>Q10 23593 (77.8%) 18.3Mb
>Q12 15882 (52.4%) 10.8Mb
>Q15 5254 (17.3%) 0.5Mb
Top 5 highest mean basecall quality scores and their read lengths
1 33.3 (22)
2 33.1 (22)
3 32.0 (21)
4 31.7 (20)
5 30.5 (20)
Top 5 longest reads and their mean basecall quality score
1 7728 (12.5)
2 7186 (11.3)
3 6587 (13.0)
4 6397 (10.4)
5 6225 (11.9)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot