NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 880.6
Mean read quality 10.3
Median read length 572.0
Median read quality 11.7
Number of reads 3,171,228.0
Read length N50 1,089.0
STDEV read length 1,752.1
Total bases 2,792,536,132.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 3165254 (99.8%) 2788.1Mb
>Q7 3032480 (95.6%) 2361.2Mb
>Q10 2362715 (74.5%) 1665.2Mb
>Q12 1410939 (44.5%) 1033.6Mb
>Q15 195484 (6.2%) 134.5Mb
Top 5 highest mean basecall quality scores and their read lengths
1 35.2 (16)
2 34.5 (16)
3 32.7 (16)
4 32.2 (16)
5 31.4 (22)
Top 5 longest reads and their mean basecall quality score
1 268902 (6.9)
2 258998 (7.6)
3 241260 (6.5)
4 239856 (7.4)
5 233319 (7.9)

Summary statistics after filtering

General summary
Mean read length 880.6
Mean read quality 10.3
Median read length 572.0
Median read quality 11.7
Number of reads 3,171,222.0
Read length N50 1,089.0
STDEV read length 1,752.1
Total bases 2,792,536,035.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 3165248 (99.8%) 2788.1Mb
>Q7 3032474 (95.6%) 2361.2Mb
>Q10 2362709 (74.5%) 1665.2Mb
>Q12 1410933 (44.5%) 1033.6Mb
>Q15 195478 (6.2%) 134.5Mb
Top 5 highest mean basecall quality scores and their read lengths
1 31.4 (22)
2 29.9 (17)
3 29.5 (25)
4 29.5 (35)
5 29.3 (16)
Top 5 longest reads and their mean basecall quality score
1 268902 (6.9)
2 258998 (7.6)
3 241260 (6.5)
4 239856 (7.4)
5 233319 (7.9)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot