NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 2,923.4
Mean read quality 8.6
Median read length 1,799.0
Median read quality 9.0
Number of reads 62,444.0
Read length N50 5,108.0
STDEV read length 3,449.9
Total bases 182,549,255.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 62321 (99.8%) 182.5Mb
>Q7 61989 (99.3%) 182.4Mb
>Q10 16866 (27.0%) 35.4Mb
>Q12 4965 (8.0%) 8.4Mb
>Q15 575 (0.9%) 0.4Mb
Top 5 highest mean basecall quality scores and their read lengths
1 31.7 (17)
2 30.9 (16)
3 29.9 (16)
4 28.9 (16)
5 28.7 (18)
Top 5 longest reads and their mean basecall quality score
1 75589 (8.1)
2 72795 (8.3)
3 64955 (8.8)
4 55300 (9.0)
5 46146 (9.1)

Summary statistics after filtering

General summary
Mean read length 2,923.5
Mean read quality 8.6
Median read length 1,799.5
Median read quality 9.0
Number of reads 62,442.0
Read length N50 5,109.0
STDEV read length 3,449.9
Total bases 182,549,222.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 62319 (99.8%) 182.5Mb
>Q7 61987 (99.3%) 182.4Mb
>Q10 16864 (27.0%) 35.4Mb
>Q12 4963 (7.9%) 8.4Mb
>Q15 573 (0.9%) 0.4Mb
Top 5 highest mean basecall quality scores and their read lengths
1 29.9 (16)
2 28.9 (16)
3 28.7 (18)
4 28.3 (16)
5 28.2 (17)
Top 5 longest reads and their mean basecall quality score
1 75589 (8.1)
2 72795 (8.3)
3 64955 (8.8)
4 55300 (9.0)
5 46146 (9.1)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot