NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 2,882.2
Mean read quality 8.8
Median read length 1,783.0
Median read quality 9.1
Number of reads 122,429.0
Read length N50 4,972.0
STDEV read length 3,332.3
Total bases 352,864,537.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 122275 (99.9%) 352.9Mb
>Q7 121761 (99.5%) 352.6Mb
>Q10 37924 (31.0%) 80.6Mb
>Q12 13091 (10.7%) 22.6Mb
>Q15 2063 (1.7%) 1.7Mb
Top 5 highest mean basecall quality scores and their read lengths
1 35.7 (16)
2 35.6 (17)
3 35.0 (16)
4 34.6 (16)
5 34.5 (16)
Top 5 longest reads and their mean basecall quality score
1 63739 (7.8)
2 53327 (9.3)
3 47603 (7.9)
4 44835 (10.2)
5 44383 (8.9)

Summary statistics after filtering

General summary
Mean read length 2,883.0
Mean read quality 8.8
Median read length 1,784.0
Median read quality 9.1
Number of reads 122,393.0
Read length N50 4,972.0
STDEV read length 3,332.4
Total bases 352,863,942.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 122239 (99.9%) 352.9Mb
>Q7 121725 (99.5%) 352.6Mb
>Q10 37888 (31.0%) 80.6Mb
>Q12 13055 (10.7%) 22.6Mb
>Q15 2027 (1.7%) 1.7Mb
Top 5 highest mean basecall quality scores and their read lengths
1 33.6 (20)
2 30.7 (24)
3 30.4 (26)
4 30.3 (48)
5 30.0 (16)
Top 5 longest reads and their mean basecall quality score
1 63739 (7.8)
2 53327 (9.3)
3 47603 (7.9)
4 44835 (10.2)
5 44383 (8.9)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot