NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 2,888.2
Mean read quality 8.8
Median read length 1,800.0
Median read quality 9.2
Number of reads 240,088.0
Read length N50 4,903.0
STDEV read length 3,297.7
Total bases 693,421,798.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 239412 (99.7%) 693.3Mb
>Q7 237744 (99.0%) 692.4Mb
>Q10 77250 (32.2%) 172.3Mb
>Q12 28112 (11.7%) 51.0Mb
>Q15 4288 (1.8%) 3.7Mb
Top 5 highest mean basecall quality scores and their read lengths
1 38.2 (16)
2 37.2 (16)
3 36.0 (16)
4 36.0 (19)
5 35.3 (16)
Top 5 longest reads and their mean basecall quality score
1 63875 (9.8)
2 58280 (9.3)
3 57832 (9.0)
4 57107 (10.3)
5 53040 (9.1)

Summary statistics after filtering

General summary
Mean read length 2,888.8
Mean read quality 8.8
Median read length 1,800.0
Median read quality 9.2
Number of reads 240,035.0
Read length N50 4,903.0
STDEV read length 3,297.8
Total bases 693,420,928.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 239359 (99.7%) 693.3Mb
>Q7 237691 (99.0%) 692.4Mb
>Q10 77197 (32.2%) 172.3Mb
>Q12 28059 (11.7%) 51.0Mb
>Q15 4235 (1.8%) 3.7Mb
Top 5 highest mean basecall quality scores and their read lengths
1 33.1 (21)
2 30.3 (21)
3 30.1 (25)
4 30.0 (21)
5 30.0 (16)
Top 5 longest reads and their mean basecall quality score
1 63875 (9.8)
2 58280 (9.3)
3 57832 (9.0)
4 57107 (10.3)
5 53040 (9.1)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot