NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 2,918.5
Mean read quality 8.6
Median read length 1,835.0
Median read quality 9.0
Number of reads 176,158.0
Read length N50 4,856.0
STDEV read length 3,265.5
Total bases 514,115,702.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 175998 (99.9%) 514.1Mb
>Q7 175626 (99.7%) 513.9Mb
>Q10 47625 (27.0%) 107.3Mb
>Q12 14844 (8.4%) 28.6Mb
>Q15 1120 (0.6%) 1.1Mb
Top 5 highest mean basecall quality scores and their read lengths
1 30.2 (16)
2 30.1 (17)
3 29.8 (17)
4 28.8 (16)
5 28.6 (17)
Top 5 longest reads and their mean basecall quality score
1 108944 (9.3)
2 72841 (9.5)
3 64286 (8.5)
4 60326 (9.9)
5 54974 (10.1)

Summary statistics after filtering

General summary
Mean read length 2,918.5
Mean read quality 8.6
Median read length 1,835.0
Median read quality 9.0
Number of reads 176,156.0
Read length N50 4,856.0
STDEV read length 3,265.5
Total bases 514,115,669.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 175996 (99.9%) 514.1Mb
>Q7 175624 (99.7%) 513.9Mb
>Q10 47623 (27.0%) 107.3Mb
>Q12 14842 (8.4%) 28.6Mb
>Q15 1118 (0.6%) 1.1Mb
Top 5 highest mean basecall quality scores and their read lengths
1 29.8 (17)
2 28.8 (16)
3 28.6 (17)
4 28.0 (17)
5 27.6 (17)
Top 5 longest reads and their mean basecall quality score
1 108944 (9.3)
2 72841 (9.5)
3 64286 (8.5)
4 60326 (9.9)
5 54974 (10.1)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot