NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 2,952.1
Mean read quality 8.5
Median read length 1,825.0
Median read quality 8.9
Number of reads 235,705.0
Read length N50 4,976.0
STDEV read length 3,334.0
Total bases 695,821,719.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 235590 (100.0%) 695.8Mb
>Q7 235242 (99.8%) 695.6Mb
>Q10 59216 (25.1%) 131.4Mb
>Q12 16668 (7.1%) 31.5Mb
>Q15 1175 (0.5%) 1.3Mb
Top 5 highest mean basecall quality scores and their read lengths
1 34.9 (16)
2 32.5 (16)
3 30.2 (16)
4 30.1 (16)
5 29.2 (17)
Top 5 longest reads and their mean basecall quality score
1 81330 (8.8)
2 72889 (9.9)
3 70270 (8.8)
4 69312 (9.7)
5 55141 (8.8)

Summary statistics after filtering

General summary
Mean read length 2,952.1
Mean read quality 8.5
Median read length 1,825.0
Median read quality 8.9
Number of reads 235,701.0
Read length N50 4,976.0
STDEV read length 3,334.0
Total bases 695,821,655.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 235586 (100.0%) 695.8Mb
>Q7 235238 (99.8%) 695.6Mb
>Q10 59212 (25.1%) 131.4Mb
>Q12 16664 (7.1%) 31.5Mb
>Q15 1171 (0.5%) 1.3Mb
Top 5 highest mean basecall quality scores and their read lengths
1 29.2 (17)
2 28.9 (16)
3 28.8 (16)
4 28.4 (16)
5 28.3 (16)
Top 5 longest reads and their mean basecall quality score
1 81330 (8.8)
2 72889 (9.9)
3 70270 (8.8)
4 69312 (9.7)
5 55141 (8.8)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot