[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H02JS.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H02DK.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H02J9.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H01RT.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H01BS.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H021E.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H00IA.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H01QP.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H01AW.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H008Q.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H027A.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H02CU.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H027V.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H00RI.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H01SU.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H014C.faa.2 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H02K5.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H0041.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H025M.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H001O.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H001Z.faa.1 for readin[hmmsearch]	21 of 69 task(s) completed at 05/23/2025 22:49:25
[hmmsearch]	28 of 69 task(s) completed at 05/23/2025 22:49:25
[hmmsearch]	35 of 69 task(s) completed at 05/23/2025 22:49:25
[hmmsearch]	42 of 69 task(s) completed at 05/23/2025 22:49:25
[hmmsearch]	49 of 69 task(s) completed at 05/23/2025 22:49:25
[hmmsearch]	56 of 69 task(s) completed at 05/23/2025 22:49:25
[hmmsearch]	63 of 69 task(s) completed at 05/23/2025 22:49:25
[hmmsearch]	69 of 69 task(s) completed at 05/23/2025 22:49:25
Results:
C:0.0%[S:0.0%,D:0.0%],F:0.0%,M:100.0%,n:148
0 Complete BUSCOs (C)
0 Complete and single-copy BUSCOs (S)
0 Complete and duplicated BUSCOs (D)
0 Fragmented BUSCOs (F)
148 Missing BUSCOs (M)
148 Total BUSCO groups searched
BUSCO did not find any match. Do not forget to check the file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/augustus.log to exclude a problem regarding Augustus
[bash]	rm: cannot remove ‘/public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/tmp/temp_genome-nextpolish_3235374942’: No such file or directory
BUSCO analysis done with WARNING(s). Total running time: 69.68585062026978 seconds
Results written in /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/

ssemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H021P.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H01B8.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H025E.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H00J8.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H01FV.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H01RF.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H00C5.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H02J4.faa.2 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H01QE.faa.2 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H00GH.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H02JR.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H02BX.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H00QI.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H02HW.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H02J2.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H015D.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H01SS.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H01WE.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H02LK.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H02AY.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H02FP.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H00EY.faa.2 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H01UZ.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H02FX.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H01MI.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H0444.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H00KS.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H007P.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H00BZ.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H028I.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H00C0.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H014M.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H01RS.faa.2 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H02E3.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H01F1.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H021G.faa.2 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H024M.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H00OD.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H01SD.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H02GL.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H00FE.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H009A.faa.1 for reading
[hmmsearch]	Error: Failed to open sequence file /public/home/zhaichaoyu/project/LR-seq/metaproject/result/PRJNA554461/SRR9687633/2.Analysis/Assemblybased/2.Polish/metaquast_out/busco_stats/run_genome-nextpolish/augustus_output/extracted_proteins/POG091H00TK.faa.2 for reading
